Evaluation of the Effect of green Tea leaf Extract on the Antibiotic Resistance of Escherichia.coli

Authors

  • Ahmed Muzahem Shallal Al-Ani1 Department of biology, college of science, University of Baghdad, Iraq .
  • Farah M. Al-Qurashi Department of biology, college of science, University of Baghdad, Iraq
  • Ali Muhsin Ali Department of biology, college of science, University of Baghdad, Iraq
  • Ali Maki Hamed Al-Dahbi Department of biology, college of science, University of Baghdad, Iraq
  • Wisam Hatem Abdullah Alrubaye Department of biology, college of science, University of Baghdad, Iraq
  • Rayaheen mohammed tamken Department of biology, college of science, University of Baghdad, Iraq
  • Doaa Abdullah Salman Department of biology, college of science, University of Baghdad, Iraq
  • Osama Abdel-Hameed Majeed Department of biology, college of science, University of Baghdad, Iraq

DOI:

https://doi.org/10.31185/bsj.Vol21.Iss38.1521

Keywords:

Green tea, Antibiotic resistance pattern, Escherichia.coli and VITEK 2 compact system

Abstract

Background: Antibiotic resistance in Escherichia coli is a major concern for public health, as it reduces options to treat infections such at urinary tract infection (UTI). Green tea (Camellia sinensis) and other natural plant products have been of interest for their ability to modify antibiotic susceptibility of bacteria, a capacity largely attributed to bioactive polyphenols. Objective: The purpose of this work was to investigate in vitro the potential effect of green tea leaf ethanol extract on the antibiotic susceptibility of E. coli isolates. Materials and Methods: Green tea leaves was extracted using ethanol and re-dissolved 1% DMSO. Five concentrations of green tea leaves ethanol extract 0.60, 0.50, 0.30, 0.16 and 0.08 g/mL were studied. E. coli isolates were detected by biochemical procedures and further tested using a VITEK 2 compact analyzer according to their biological profile; susceptibility to different antibiotic types was also established. Results: Exposure to green tea leaf ethanol extract at 0.16g/ml and 0.08 g/mL concentrations was associated with notable changes in the antibiotic susceptibility profile of E. coli. The observed alterations included a shift from a generally susceptible profile to an extensively drug-resistant (XDR) phenotype under the experimental conditions. Conclusion: Findings suggest that green tea leaf ethanol extract may modulate E. coli antibiotic resistance profile in vitro, indicating potential relationship between plant compounds and microbial antibacterial resistance mechanisms necessitating additional exploration

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Published

2026-03-01

Issue

Vol. 21 No. 38

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Copyright (c) 2026 م.م. أحمد مزاحم شلال كلية العلوم/جامعة بغداد/قسم علوم الحياة، م. د.فرح محمد صالح تركي كلية العلوم/جامعة بغداد/قسم علوم الحياة، م. علي محسن علي كلية العلوم/جامعة بغداد/قسم علوم الحياة، ، م.م.علي مكي حميد كلية العلوم/جامعة بغداد/قسم علوم الحياة، م.م. وسام حاتم عبد الله كلية العلوم/جامعة بغداد/قسم علوم الحياة، م.م.رياحين محمد تمكين كلية العلوم/جامعة بغداد، م.م. دعاء عبد الله سلمان كلية العلوم/جامعة بغداد، م. د.أسامة عبد الحميد كلية العلوم/جامعة بغداد/قسم علوم الحياة

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