Patterns of PPARG, IRS1, TCF7L2, and KCNJ11 Genes in Obese Diabetic and Non-Diabetic Individuals
DOI:
https://doi.org/10.31185/bsj.Vol22.Iss43.1664Keywords:
Keywords: rs1801278, rs5219, rs1801282, and rs7903146, Gene polymorphism, IraqAbstract
Obesity is a complex multifactorial condition influenced by both environmental and genetic factors, resulting in various complications such as type2 diabetes mellitus (T2DM). However, phenotypic variations lead to increasing the interest about the role of genetic polymorphisms in modulating susceptibility to T2DM among obese individuals. This study aims to molecular identification the expression and risks of IRS1, KCNJ11, PPARG and TCF7L2 genes among the obese individuals who having the T2DM (OT2DM) and non-diabetic (OND) with investigation the levels of lipid profiles and relationship to some demographic risk data. Materials and methods: Totally, 90 individuals (45 OT2DM and 45 were selected and subjected to collection of venous blood that examined molecularly and serologically. Molecular genotyping using allele-specific PCR-RFLP was served for SNPs targeting rs1801278 for IRS1 while T-ARMS PCR was utilized for SNPs targeting rs5219 for KCNJ11, rs1801282 for PPARG, and rs7903146 for TCF7L2 genes. The findings IRS1 G>A (rs1801278) were revealed a marked elevation in frequency and risk of GA and AA genotypes of OT2DM individuals when compared to OND population. In OT2DM, there was a significant higher frequency and risk of A allele than G allele. Regarding KCNJ11 C>T (rs5219), the frequency and risk of CT genotype was apparently higher than the CC and TT in OT2DM than OND. Subsequently, the OT2DM was reported a higher frequency and risk of T allele than C allele. Concerning PPARG G>C (rs1801282), values of frequency and risk of GC and CC genotypes were elevated more than the GG genotype among the OT2DM population; whereas, results of allele frequency in OT2DP were detected a marked elevation in existence and risk of C allele in comparison to G allele. Relation TCF7L2 C>T (-rs7903146), our findings identified that the frequency and risk of CT and TT genotypes in OT2DM population was significantly higher than OND. Subsequently, the percentage and risks of T allele in OT2MD population was markedly higher the C allele. In conclusion, this study indicates the predominant and risk of IRS1 G>A (rs1801278), KCNJ11 C>T (rs5219), PPARG G>C (rs1801282), and TCF7L2 C>T (-rs7903146) in OT2DM compared to OND suggesting importance of furthermore studies to investigate the roles of various genes in OT2DM, and possible utilization of such genes in determination of such metabolic disorders.
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