Histopathological and Immunohistochemical Study of Human Placenta in First and Second Trimester of Pregnancy Associated with Spontaneous Abortion in Wasit Governorate
DOI:
https://doi.org/10.31185/bsj.Vol23.Iss45.1815Keywords:
الكلمات المفتاحية: التغيرات النسيجية المرضية، المشيمة، التقنية المناعية الهيستوكيميائية، محافظة واسط، فقدان الحمل.Abstract
Immunological variables during pregnancy are associated with recurrent pregnancy loss of unexplained origin. This study aims to investigate the immunological and cellular roles of placental cells to determine whether their cellular activity is linked to an increased risk of miscarriage during the first and second trimesters. 32 placental tissue samples were collected from women who had experienced spontaneous abortion in Wasit Governorate and prepared histologically using hematoxylin and eosin staining to study general changes. In addition, an immunohistochemical technique using anti-CD68 was used to determine macrophage expression levels. The results revealed distinct histological changes in the affected villi and placental cells, coinciding with a marked increase in macrophage immunoreactivity especially in the second trimester of miscarriage. This leads us to conclude that the immune and inflammatory response plays a significant role in miscarriage. Moreover, the work contributes to clarifying the connection between immunohistochemical changes and the mechanism of pregnancy loss, which may enable us in the future to develop better diagnostic and therapeutic methods for preserving pregnancy.
References
References
1. Camayo, F. J. A., Martins, L. A. B., & Cavalli, R. C. (2011). Perda gestacional retida: Tratamento baseado em evidência. Femina, 39(1), 49–56.
2. Kolben, T. M., Rogatsch, E., Hester, A., Kuhn, C., Schmoeckel, E., Czogalla, B., et al. (2019). Involvement of ILR4α and TLR4 in miscarriages. Journal of Reproductive Immunology, 131, 36–43. doi: https://doi.org/10.1016/j.jri.2018.12.001
3. Sadler, T. W. (2012). Fetal membranes and placenta. In Langman’s medical embryology (12th ed., pp. 96–101). Wolters Kluwer.
4. Lanir, N., Aharon, A., & Brenner, B. (2003). Haemostatic mechanisms in human placenta. Best Practice & Research Clinical Haematology, 16, 183–195. doi: https://doi.org/10.1016/S1521-6926(02)00098-1
5. Fox, H. (1999). Development of placenta and membranes. In J. Dewhursts, M. Deswiet, & G. Chamberlain (Eds.), Basic sciences in obstetrics and gynecology. Churchill Livingstone.
6. Vijay, V. (1994). Handbook of placental pathology (2nd ed.).
7 Christopher, P. C. (2004). The female genital tract. In R. S. Cotran, V. Kumar, & T. Collins (Eds.), Pathologic basis of disease (7th ed., pp. 1079–1080). WB Saunders.
8. Roberts, D. J., & Oliva, E. (2006). Clinical significance of placental examination in perinatal medicine. Journal of Maternal-Fetal and Neonatal Medicine, 19, 255–264. doi: https://doi.org/10.1080/14767050600676349
9. Bancroft, J. D., & Layton, C. (2019). The hematoxylins and eosin. In K. S. Suvarna et al. (Eds.), Bancroft’s theory and practice of histological techniques (8th ed., pp. 126–138). Elsevier.
10. Kiernan, J. A. (2015). Immunohistochemistry. In Histological and histochemical methods: Theory and practice (4th ed., pp. 454–490). Scion Publishing.
11. Ermis, I. S. (2021). Losartan protects ovarian tissue against ischemia-reperfusion: An immunohistochemical study. Analytical and Quantitative Cytopathology and Histopathology, 43(5), 345–352.
12. Tasin, C., Ermiş, I. S., & Deveci, E. (2021). Endothelin-1 and APAF-1 expression in the umbilical cord of placenta previa cases. Analytical and Quantitative Cytopathology and Histopathology, 43(5), 439–445.
13. Hein, A. L., Mukherjee, M., Talmon, G. A., Natarajan, S. K., Nordgren, T. M., Lyden, E., Hanson, C. K., Cox, J. L., Santiago-Pintado, A., Molani, M. A., et al. (2021). QuPath digital immunohistochemical analysis of placental tissue. Journal of Pathology Informatics, 12, 40.
14. Porter, R. J., Din, S., Bankhead, P., Oniscu, A., & Arends, M. J. (2023). QuPath algorithm accurately identifies MLH1-deficient inflammatory bowel disease-associated colorectal cancers in a tissue microarray. Diagnostics, 13, 1890.
15. Al-Khazraji, H. A., et al. (2023). A comparative study of the diagnosis of Toxoplasma gondii in human placenta by traditional method, restriction fragment length polymorphism, and the immunohistochemistry method. The Egyptian Journal of Hospital Medicine, 90, 1707–1712. doi: https://doi.org/10.21608/EJHM.2023.284278
16. Khong, T. Y., Mooney, E. E., Ariel, I., Balmus, N. C. M., Boyd, T. K., Brundler, M. A., et al. (2016). Sampling and definitions of placental lesions: Amsterdam placental workshop group consensus statement. Archives of Pathology&Laboratory Medicine, 140, 698. doi: https://doi.org/10.5858/ARPA.2015-0225-CC
17. Stallmach, T., Hebish, G., Meter, K., Dudenhausen, J., & Vogel, M. (2001). Rescue by birth: Defective placental maturation and late fetal mortality. Obstetrics&Gynecology, 97, 505–509.
18. Habek, D. (2011). Multiple intraplacental hematomas—Kline’s hemorrhage: Case report. Acta Clinica Croatica, 50, 423–425.
19. Sandoval, O. (2016). The placenta in a case of pregnant woman infected by Chikungunya virus.
20. Petersen, O. M., Heller, D. S., & Joshi, V. V. (2006). Handbook of placental pathology. Taylor & Francis.
21. Kraus, F. T. (2013). Fetal thrombotic vasculopathy: Perinatal stroke, growth restriction, and other sequelae. Surgical Pathology Clinics, 6, 87–100. doi: https://doi.org/10.1016/J.PATH.2012.10.001
22. Rebutini, P. Z., Zanchettin, A. C., Stonoga, E. T. S., Prá, D. M. M., de Oliveira, A. L. P., Dezidério, F. S., et al. (2021). Association between COVID-19 pregnant women symptoms severity and placental morphologic featu
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